Dr Richard Webb 

Contact Details:
Room T2.16
Cardiff School of Health Sciences
University of Wales Institute, Cardiff (UWIC)
Western Avenue, Cardiff CF5 2YB, UK
(Tel: + 44 (0) 29 2041 6844;
Fax: + 44 (0) 29 2041 6982 ;
Email. rwebb@uwic.ac.uk)

Present Appointment (since 2003)
Principal Lecturer in Biomedical Sciences/
First-Year Tutor, BSc (Hons) Biomedical Sciences,
Cardiff School of Health Sciences,
Llandaff Campus,
University of Wales Institute Cardiff,
Cardiff CF5 2YB

Career details

a) Post-doctoral Research Officer, Department of Biology & Biochemistry, University of Bath, BATH BA2 7AY (April 2001-April 2003).
b) Post-doctoral Research Scientist, Department of Infectious Diseases, St. George’s Hospital Medical School, University of London. LONDON SW15 0RE (October 1999 – April 2001).
c) Post-doctoral Research Fellow, Division of Biochemistry & Molecular Biology, School of Biological Sciences, University of Southampton SO16 7PX (March 1996 – September 1999).
d) Several months’ volunteer work overseas as Resident Biologist in the Tambopata/Candamo Nature Reserve, Peru, where I participated in a variety of biological studies that were being undertaken within the reserve, and also led nature hikes, wrote educational literature and gave informal seminars while acting as specialist naturalist guide to ecotourists (May - September 1995).

Degrees:

  • PhD (Medical Biochemistry) - University of Wales College of Medicine. (1995)
  • (Thesis entitled: “”Functional Characterization of Two Epithelial ATP-binding Proteins: the ER Ca2+,Mg-ATPase and the Cystic Fibrosis Gene Protein.”)
  • B.Sc. (II[i] Hons Biochemistry) – Liverpool University (1990).
  • Dip.Bio.Med. – University of Wales College of Medicine (1991).
  • Post-Graduate Certificate of Education (Post-Compulsory Education and Training) - University of Wales Institute, Cardiff (2003-5).

Research Interests
I have extensive experience in the investigation of cellular transport and signalling processes (particularly those involving Ca2+), as applied to the pathophysiology of several diseases. 
My PhD involved functional characterisation of the ATP-binding proteins CFTR and SERCA2b. As a post-doctoral research scientist, I investigated the skeletal muscle Ca2+ pump enzyme SERCA1, whose dysfunction is linked to Duchenne Muscular Dystrophy (Southampton University, 1996-1999); the functional characterisation of malarial transport proteins via heterologous expression in vertebrate cells (St George’s Hospital Medical School, London, 1999-2001); and several different signalling/transport proteins in cardiovascular disease (Bath University, 2001-2003).

Since my arrival in UWIC in 2003, I have integrated the expertise gained earlier in my career into the ‘Diabetes and Metabolic Health’ group’s research into the inflammatory and signalling aspects of diabetes and its complications, especially the effect of exercise, dietary regimes and therapeutic interventions in triggering signaling events relevant to diabetes, obesity and cardiovascular disease.

PhD Supervision
Ph.D. Supervision Completions: Neenu Singh: “Rosiglitazone-induced PPAR-independent and PPAR-dependent anti-inflammatory effects in monocytic cells” – October 2003-April 2007.

Ph.D. Supervisions (ongoing):
Joanne Caddy: “The Non-Genomic Effects of the PPAR Ligand Rosiglitazone on [Ca2+]i in mammalian monocytic and smooth muscle cells” – Oct 2005 onwards.

Hannah Moir: “AMPK Inactivation in Mononuclear Cells: A Potential Intracellular Mechanism For Exercise-Induced Immunosuppression” – Jan 2006 onwards.

Craig Sims: “The Role of Mitochondria in Endothelial Cell Senescence” – Oct 2006 onwards.

Lowri Mainwaring: “Dietary lipids as PPAR ligands and regulators of inflammation” – Oct 2007 onwards.

Lisa Atkin: “Does treatment with PPAR Ligands constitute a cellular stress?” – Oct 2007 onwards.

Selected Publications
Butcher, L., Backx, K., Roberts, A., Thomas, A., Webb, R., and Morris, K. (2008): “Low-intensity exercise, LDL oxidation and regulation of lipoprotein clearance genes.” Med. Sci. Sports. Ex. [in press]

Atkin, L. and Webb, R. (2008): “Rosiglitazone-Induced SERCA2b Inhibition: Implications for Monocyte Cytoskeletal Remodeling and Diabetic Microangiopathy” Bioscience Horizons 1, p.1-8.

Caddy, J., Singh, N., Atkin, L., Ahluwalia, M., Roberts, A.W., Lang, D., Thomas, A.W. and Webb, R. (2008): "Rosiglitazone Transiently Disturbs Calcium Homeostasis in Monocytic Cells" Biochemical Biophysical Research Communications 366, p.149-155.

Moir, H., Butcher, L., Jones, K.P., Hughes, M.G., Neale, H., Jia, H., Al-Ismaily, Z., and Webb, R. (2008): "AMPK Inactivation in Mononuclear Cells: A Potential Intracellular Mechanism For Exercise-Induced Immunosuppression." Applied Physiology, Nutrition and Metabolism 33, p.75-85.

Singh, N., Webb, R., Adams, R., Evans, S-A., Al-Mosawi, A., Evans, M., Roberts, A.W., Thomas, A.W. (2005) – “The PPAR activator, Rosiglitazone, inhibits actin polymerisation in monocytes: involvement of Akt and intracellular calcium” Biochem. Biophys. Res. Comm. 33, p.455-462.

Eckstein-Ludwig, U., Webb, R.J., Van Goethem, I.D.A., East, J.M., Lee, A.G., Kimura, M., O’Neill, P.M., Bray, P.G., Ward, S.A. & Krishna, S. (2003) - “Artemisinins target the SERCA of Plasmodium falciparum” Nature 424, pp. 957 – 961.

Krishna, S. Woodrow, C.J., Webb, R., Penny, J., Takeyasu, K., Kimura, M. & East, J.M. (2001) – “Expression and Functional Characterization of a Plasmodium falciparum Ca2+-ATPase (PfATP4) Belonging to a Subclass Unique to Apicomplexan Organisms” J. Biol. Chem. 276, pp. 10782-7.

Webb, R., Khan, Y.M., East, J.M. & Lee, A.G. (2000) – “The Importance of Carboxyl Groups on the Lumenal Side of the Membrane for the Function of the Ca2+-ATPase of Sarcoplasmic Reticulum” J. Biol. Chem. 275, pp. 977-82.

Webb, R., East, J.M., Sharma, R.P. & Lee, A.G. (1998) – “Hydrophobic Mismatch and the Incorporation of Peptides into Lipid Bilayers: a Possible Mechanism for Retention in the Golgi” Biochemistry 37, pp. 673-9.

Webb, R. & Dormer, R.L. (1995) – “Photoaffinity Labelling of the ATP-binding Sites of 2 Ca2+,Mg-ATPase Isoforms in Pancreatic Endoplasmic Reticulum” Biochim. Biophys. Acta 1233, pp.1-5.

Dormer, R.l., Capurro, D.E., Morris, M.R. & Webb, R.J. (1993) – “Demonstration of 2 Isoforms of the SERCA2b-type Ca2+,Mg-ATPase in Pancreatic Endoplasmic Reticulum” Biochim. Biophys. Acta 1152, pp. 225-30.